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La gamma-butirrobetaina viene poi idrossilata dalla gamma butirrobetaina idrossilasi (un enzima legante di) in L-carnitina, che richiede ferro sotto forma di Fe2+
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As expected at pH 7.4, SPA was considered deprotonated on both the sulfonate (SO 3 ) and carboxylate (COO ) groups, with a net charge of -2