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Specifically, the purified SOD1 proteins were reconstituted with metals by an overnight dialysis against buffer A (50 mM Tris-HCl, pH 7.5, 0.1 M NaCl, and 10% glycerol) supplemented with ZnSO 4 and CuSO 4 (1 mM each)

In: GlickMGreenbergMSLockhartPBChallacombeSJ, editors

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Taking it with food also serves a secondary purpose: it significantly reduces the likelihood of mild gastrointestinal discomfort, which is the most commonly reported side effect of glucosamine sulfate