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DNA was extracted from the homogenized tissue using a modified cetyl trimethylammonium bromide (CTAB) protocol using chloroform:isoamyl alcohol 24:1 (Fulton et al., 1995)

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Short-term handling at room temperature during active laboratory use is generally acceptable, but the lyophilized material should be returned to frozen storage promptly to preserve research-grade integrity
Therefore, it is crucial to prevent and treat NAFLD at any possible stage
