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Additionally, the average background noise of unmodified A sites was as low as 0.49%, which is 10 times lower than the cutoff ( 10) were selected for further analysis
Fixed organs were then transferred to a 30% sucrose solution overnight and then embedded in optimal cutting temperature (OCT) compound (Tissue-Tek Cat#4583), followed by subsequent cryosection into 5-10 m sections prior to confocal imaging and antibody staining
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