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These complex structures provide the first information regarding the location of the ascorbate and GSH binding sites and their interacting residues
Among various alkylating agents, N-ethylmaleimide is identified as the most reliable for thiol stabilization in liquid chromatographymass spectrometry (LC-MS) workflows, while specific reagents such as monobromobimane or -(4-hydroxyphenyl)ethyl iodoacetamide (HPE-IAM) are required for persulfide and polysulfide detection
Appropriate supplementation or an IV administered by a doctor remain the best ways to boost glutathione levels
