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Jolliffe DA, Ganmaa D, Wejse C, Raqib R, Haq MA, Salahuddin N, Daley PK, Ralph AP, Ziegler TR, Martineau AR

Macrophage culture and stimulation For murine BMDM isolation, total cells from the bone marrow were plated at 7.0 10 5 /mL for 57 days in complete Dulbeccos modified Eagle medium (DMEM) (Gibco #11965) supplemented with 2 mM Glutamine, 10% FBS, 100 U/mL penicillin/streptomycin and 20 ng/ml mouse M-CSF allowing for BMDM differentiation and growth 3

However, significant recovery (30%50%) was observed with the Nrf2 protein in both tissues of GK rats

NAD-driven metabolic activity increases the need for antioxidant protection
