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Next, a 20 L reaction mixture containing 1 M RNA, 1 PKN buffer, 4 M of each Rbs-VQ precursor, and 1 mM EDTA was prepared

Peer WA, Mahmoudian M, Freeman JL, Lahner B, Richards EL, Reeves RD, Salt DE (2006) Assessment of plants from the brassicaceae family as genetic models for the study of nickel and zinc hyperaccumulation

doi: 10.1111/j.1467-7652.2011.00596.x

It was propagated in 3D hydrogel
