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The samples were vortexed 15 s and left in a freezer at -20 C for 60 min, followed by centrifugation at 21,100 RCF for 15 min at 4 C
doi: 10.1016/j.ajog.2017.01.021
With the limited high-level evidence studies on each host metabolic category, we could only conclude that the considerations of whether to take supplementation varied by the individuals metabolic status and their nutrient reserves
