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Lignans are widely distributed in the plant kingdom, and they exist in plant roots, stems, rhizomes, leaves, fruits, flowers, seeds, resins, and xylem

Real-time quantitative PCR Real-time quantitative PCR (RT-qPCR) was performed using a StepOnePlus Real-time PCR system (Applied Biosystems, Foster City, CA, USA) according to the manufacturers protocol with SYBR Premix Ex Taq (Takara Bio Inc., Shiga, Japan)

Each experiment was conducted independently three times with duplicate measurements to ensure reproducibility and statistical robustness [1]

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