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The 10dilution buffer (10% Triton x-100, 1 M NaCl, 1% Na-Deoxycholate, 5% N-Lauroylsarcosine, 5 mM EGTA) was added to the lysate, samples were centrifuged at maximum speed at 4 C for 20 min, and the following antibodies were added: -GFP (Santa Cruz, sc-9996, 5 g per sample), -BRD4 (Fortis, A700-004, 5 g per sample), -ASXL1 (made inhouse, 40 L anti-sera per sample), or -BAP1 (made inhouse, 40 L anti-sera per sample)

Cholesterol Efflux Is Differentially Regulated in Neurons and Astrocytes: Implications for Brain Cholesterol Homeostasis. Biochimica et Biophysica Acta 1831: 263275

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Like other classical LMW thiols, BSH is readily oxidized to bacillithiol disulfide (BSSB)