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For co-staining with antibodies, EdU-labeled gonads were incubated in the primary antibodies after washing for three times, and subsequently in the secondary antibodies as described above

Interestingly, a recent study has identified ERMCSs as required for lipid radical transfer from mitochondria to the ER to reduce mitochondrial oxidative stress 88 , which could represent an alternative mechanism for ERMCS-localised GLP-1R signalling to enhance -cell survival under glucolipotoxic conditions

Durability: Weight often returns after stopping treatment, especially without lifestyle and coaching support

References: Kapoor E, Collazo-Clavell ML, Faubion SS