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The mucosal samples were rapidly frozen in liquid nitrogen and then stored at 80 C until analysis
Studies have shown that pretreatment with API (20 mg/kg) significantly improves the levels of hepatic antioxidant markers (GSH, CAT, GPX and SOD) in mice with MTX-induced liver injury, and reduces levels of liver function indicators (AST, ALT and ALP) and inflammatory factors (TNF- and IL-1) (Goudarzi et al., 2021)
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